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• RtattudIAzie Ar To /Valuate the efficacy of using alkaline hydrogen peroxide (All?) to incfease Ng digesHbility of structural ctirbohydrates In tree leaves. , 2. To identify and characterize chemical changes that take place In AliP treated tree leaves and estimate to what degree such changes affect ruminal digestibility of protein and fiber. rrojesidaistu • Object'v'e 1: This experiment will compare digestibility and utilization of four diets based on untreated br treated tree leaves by sheep. Arm treated tree leaves are prepared by an extrusion process In which a sodium hydroxide solution is injected onto leaves ea they pass through the extruder chamber. pH of the leaves is elevated to 11.5. Hydrogen petokide ft then injected into the alkali tree leaves and the mixture forced through the extruder die. The four diets differ principally in the level of untreated or ARP treated leaves. The treatments will be: (a) 70% untreatbd tree leaves, 30% corn-soy-mineral concentrate, , I . (b) 30% untreated tree leaves, 70% corn-soy-mineral concentrate, (c) 70% AtIP treated tree leaves, 30% corn-soy-mineral concentrate or (d) 30% A1113 treated tree , leaves, 70% corn-soy-mineral concentrate. Ruminally cannulated sheep will be utilized in a 4 x 4 Latin square statistical design. Each sheep will be fed each diet for a Period of 21 days. Ruminal dIgesta will be collected during the last six days of each per' ' and analyzed for pH, ammonia and volatile fatty acid concentration. Fecal fiv sam es will be collected and analyzed for dry matter, organic matter, crude protein, NDF, ADF and lignin. Digestibility of organic matter, crude protein and fiber will be calculated and compared. Objective 2. Previous work in our laboratory indicates that ARP treatment greatly Increases the fraction of tree leaves that is water soluble. The intent of this experiment is Ito determine if the soluble portion of treated tree leaves has any affect , - on ruminal protein or fiber digestion. ARP and untreated tree leaves will be refluxed in distilled water to dissolve the water soluble water soluble fraction. Residue will be separated by filtration. Filtrate will be analyzed to determine the presence of phenolic compounds (Reed et al 1985). i Samples of alfalfa protein, casein, and soy protein will then be suspended in the 1 i filtrate. Water will then be removed by drying under low heat. Rate and extent of i 'totem» degradation of each of the treated proteins and of untreated proteins will hen by estimated by an in vitro technique (Broderick, 1987). 'he effect of the water soluble component of ARP treated tree leaves on ruminal iber digestion will be assessed by incubating a standard legume (alfalfa) or a [andard grass (brome) in batch cultures of buffered rumen fluid that contain 0, 1, 5, Clor .